Project R-7828

Profiling stem-cell-specific activities in an in vivo biocompatibility study of mesoporous silica nanoparticles (Research)

There is very little focus on the potential toxicity of manufactured nanoscale materials. Nevertheless, their toxicological profile may differ from materials of similar composition because of their small size and unique physicochemical properties. Mesoporous silica nanoparticles are an example of nanoscale material with extensive use in biomedical applications. Although considered safe for prolonged internalization in cells, several studies indicate cytotoxic effects in different cell types. In this project, we will assess the biocompatibility of silica nanoparticles, thereby focusing on their impact on stem cell functioning. Stem cells are an important target of several biomedical applications for which these nanoparticles are currently used. Planarians (flatworms with a high regenerative capacity) offer the possibility to assess stem cell responses in vivo, thereby taking into account the cellular niche. The kinetic and dynamic profiles of silica nanoparticles with a different composition will be evaluated, including single cell responses. The latter is highly relevant in toxicological research, as important responses can be masked due to tissue heterogeneity. By integrating these aspects in our study, as well as by investigating potential effects at different biological levels, we aim to profile the biological activity of silica nanoparticles. Moreover, the results of this study will aid in assessing the safety of these nanoparticles.

01 January 2017 - 31 December 2021