Project R-14704

Extreme low-frequency-electromagnetic stimulation (ELF-EMS) as an innovative tool to target endothelial cells in ischemic stroke (Research)

Ischemic stroke is a neurological disorder caused by a sudden disrupted blood supply of the brain. It is the second leading cause of death worldwide and often leads to permanent disabilities. Only a small proportion of patients can be adequately treated, highlighting the urgent need for new treatments. Recently, our laboratory discovered that 'extreme low-frequency-electromagnetic stimulation (ELF-EMS)' applied in the acute phase reduces brain damage in a mouse model of ischemic stroke. This effect was caused by stimulation of the brain blood flow. There is currently no information on how exactly LF-EMS activates blood vessels. The first aim of the project is to further improve ELF-EMS treatment by optimizing its duration, frequency and flux in mouse models. This will be achieved by using functional ultrasound imaging, allowing the assessment on the brain in awake mice. Secondly, we will unravel how exactly the ELF-EMS activates the endothelial cells, which form the blood vessels. This will be achieved by using different innovative techniques ranging from advanced gene and protein analysis as well as targeted deletion of putative involved intracellular mediators. Thirdly, we will also investigate if ELF-EMS also affects long-term repair after ischemic stroke . Insights obtained in this project will enhance our current knowledge of LF-EMS as well as pave the way for potential clinical trials of this 'electroceutical'.

01 January 2024 - 31 December 2027