Project R-14358

Unveiling molecular interactions through temperature-controlled microfluidic devices and fluorescence microscopy (Research)

Precision plays a crucial role in the success of drug therapy, as it enables optimal treatment outcomes while minimizing undesirable side effects. At its core, precision entails the targeted interaction of molecules, like puzzle pieces fitting together. To study these interactions, microfluidic devices or 'micro labs' are designed. These devices enable the exploration of the intricate world of small molecules such as DNA and proteins. Moreover, these micro labs offer an exceptional capability – precise temperature control. The molecules of interest are carefully introduced into the micro lab, where the temperature is finely tuned. The level of temperature precision is analogous to the nuanced adjustments made on a stovetop but at a much smaller, molecular scale. This innovation opens up a realm of possibilities for comprehending how these minuscule building blocks of life interact with one another by introducing small temperature variations. To visualize the effect of the small temperature changes on the molecular behavior, a specialized tool is employed – the fluorescence microscope. This instrument allows us to closely monitor and comprehend the intricate molecular interactions transpiring inside these micro labs by studying fluorescent (light-emitting) probes. Monitoring alterations in the intensity of these light signals directly reveals insights into molecular interactions, including the coming together or the separation of specific molecules. In essence, these temperature-controlled micro labs, coupled with fluorescence microscopy, act as a powerful magnifying glass, offering us a window into the captivating world of tiny molecule interactions. This knowledge bears significant implications, particularly in the field of medicine. It holds the potential to drive the development of more precise and effective medicines, especially those tailored to target specific diseases.

01 October 2023 - 30 September 2025