Project R-15138

A hidden threat to inflammatory bowel disease: how micro- and nanoplastics affect the foundations of the gut barrier - a study in human intestinal models (Research)

We eat small plastic particles everyday, but can they damage our gut? And if so, how is this related to the development or exacerbation of inflammatory bowel diseases? These questions can only be solved if we know which properties of micro- and nanoplastics (MNPs, <5 mm), adversely affect intestinal barrier functioning, and how they induce these effects. In the current project, I employ a multidisciplinary approach, integrating toxicology, (nano)physics, immunology and gastroenterology to investigate to what extent intestinal barrier function is related to uptake and intracellular localisation of MNPs, and how this depends on their physicochemical properties. MNPs of different shapes, sizes and composition will be synthesized to mimic the particles found in nature. Uptake and intracellular localisation of MNPs will be studied with state of the art imaging techniques. Underlying cellular mechanisms will be studied in-depth in advanced triculture intestinal models. A 2D-intestinal organoid model will be employed to get insight in the role of innate immune responses and cell-to-cell communication. This study will provide thorough understanding how intestinal cells respond and interact upon exposure to environmentally relevant MNPs. Furthermore, it offers in-depth insight into the interplay between MNPs and intestinal pathologies. Overall, it will provide key information for the plastics industry to develop products from a safe-by-design approach.

01 November 2024 - 31 October 2027