qPCR training - 2024

During the last decades, the number of researchers from different disciplines performing transcriptome analysis has increased dramatically. Despite the use of state-of-the-art high throughput technologies for whole genome transcriptome profiling (e.g. next generation sequencing or microarray), quantitative real-time PCR (qPCR) remains the gold standard for targeted gene expression analysis due to its superior sensitivity, specificity, linear dynamic range of quantification and a high level of flexibility.

A qPCR experiment involves several steps ranging from experimental design, RNA extraction, assay design, data generation to data analysis. While the practical performance of a real-time PCR quantification experiment is relatively straightforward, it is important to identify critical steps that may impact the quality and robustness of the experiment. Moreover, many researchers experience a genuine need for more in-depth training of their experiment design and data-analysis skills in order to generate biologically relevant and reliable results.

To accommodate these needs Outside The Lines offers a qPCR-focused course in which the fundamental and advanced principles of experiment design, sample and assay quality control and data analysis are covered.

Learning outcomes

At the end of the course, you will have learned more about:

• Experimental design
• Sample preparation and quality control
• Assay design and validation
• qPCR amplification
• Absolute quantification
• Relative quantification
• Data quality and control
• Bio-statistical analysis

Competences

An important part of preparing for any further professional step is becoming (more) aware of the competences you have developed and/or want to develop. In the current workshop, the following competences from the UHasselt competency overview are actively dealt with:

• academic research competences:
  • research methods
  • subject knowledge
• task-orientedness:
  • quality assurance

For whom?

• PhD students
• 20 places available

When and where?

• May 16 & 17, 2024 - 09:00-17:00
• Campus Diepenbeek, building D, room H5

Details?

• You will be contacted beforehand to install the qbase+ software on your (Windows) laptop

Registration?

• Registration is possible here from February 08 until April 24, 2024.
• As places are limited, registering does not automatically imply that you will be able to participate. You will be notified by email in due time.
• Please cancel your registration at least one week in advance in case you cannot make it (cf. cancellation & no-show policy).

Acknowledged as?

• DS H&LS: category 'scientific skills' - one compulsory elective technical/methods courses
• DS S&T: category 'advanced discipline-specific knowledge' - one advanced course