Technology
In our Lab, the clocks tick every picosecond, and the rulers have an Ångstrom scaling. We specialize in quantitative fluorescence methods that allow measuring the dynamics of biological complexes in detail. Recently, we've also specialized in microfabrication and microfluidics, and have successfully set up a protein production and labeling pipeline.
Diffusion and Binding
Biomolecules hide a wealth of information in the way they move about! We specialize in fluctuation spectroscopy methods for quantifying biomolecular diffusion and binding processes.
Conformation
Biomolecules hide a wealth of information in the way they move about! We specialize in single-molecule methods for quantifying biomolecular structure and conformational dynamics.
Time-resolved fluorescence
Fluorophores hide a wealth of information in the way they fluoresce! We specialize in time-resolved methods for quantifying fluorescence lifetimes and anisotropy.
ZEISS Spectral RICS
Zoom in on what biomolecules are actually doing inside cells! Thanks to a software wizard workflow and automated analysis, multicolor crosstalk-free raster image correlation spectroscopy has never been easier!
Microfabrication & microfluidics
We generate our own microfluidics and microstructures using electron and optical lithography and microfluidics pumps.
Protein Production
We have a fully operational bacterial protein production pipeline including FPLC-based purification. We label single-molecule quality pure protein batches using fluorophores.
Assymmetric Motion and Flow
Explore molecular motion beyond Brownian diffusion using array-based confocal detection.
Equipment
At the Dynamic Bioimaging Lab, there's microscopes for everyone and everything...
- We house both widefield, confocal and light-sheet systems,
- We image single molecules, cells, organoids, organisms and small-animal organs,
- We record data from the picoseconds to real time,
- We go from the Angstrom to centimeter length scale,
- We analyse steady state or fluctuating fluorescence signals, and
- We record label-free and spectrally resolved samples.
The Advanced Optical Microscopy Centre web page lists the different instruments.
Contact
prof. dr. Jelle HENDRIX
Agoralaan C (BIOMED), B3590 Diepenbeek
Microscopy Facility
Business Development
Agoralaan C (BIOMED), B3590 Diepenbeek